Grafted biomembranes containing membrane proteins--the case of the leucine transporter.
نویسندگان
چکیده
Here, we bind the sodium dependent amino acid transporter on nitrilotriacetic acid/polyethylene glycol functionalized gold sensors in detergents and perform a detergent-lipid exchange with phosphatidylcholine. We characterize the LeuT structure in the adsorbed film by magnetic contrast neutron reflection using the predicted model from molecular dynamic simulations.
منابع مشابه
Incorporated Poly Acrylic Acid-co-Fe3O4 Nanoparticles Mixed Matrix Polyethersulfone based Nanofiltration Membrane in Desalination Process
Polyethersulfone (PES) based nanocomposite nanofiltration membrane was prepared by immersion precipitation method and casting solution technique using poly (acrylic acid) grafted-iron oxide nanoparticles (Fe3O4) as hydrophilic filler additives. For this purpose, iron oxide nanoparticles were modified by in situ polymerization of acrylic acid in aqueous solution by potassium persulfate as initi...
متن کاملBuilding an understanding of cystic fibrosis on the foundation of ABC transporter structures.
Cystic fibrosis (CF) is a fatal disease affecting the lungs and digestive system by impairment of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR). While over 1000 mutations in CFTR have been associated with CF, the majority of cases are linked to the deletion of phenylalanine 508 (delta F508). F508 is located in the first nucleotide binding domain (NBD1) of CFTR. This mutation is...
متن کاملSingle Point Mutations Result in the Miss-Sorting of Glut4 to a Novel Membrane Compartment Associated with Stress Granule Proteins
Insulin increases cellular glucose uptake and metabolism in the postprandial state by acutely stimulating the translocation of the Glut4 glucose transporter from intracellular membrane compartments to the cell surface in muscle and fat cells. The intracellular targeting of Glut4 is dictated by specific structural motifs within cytoplasmic domains of the transporter. We demonstrate that two leuc...
متن کاملIst2 in the Yeast Cortical Endoplasmic Reticulum Promotes Trafficking of the Amino Acid Transporter Bap2 to the Plasma Membrane
The equipment of the plasma membrane in Saccharomyces cerevisiae with specific nutrient transporters is highly regulated by transcription, translation and protein trafficking allowing growth in changing environments. The activity of these transporters depends on a H(+) gradient across the plasma membrane generated by the H(+)-ATPase Pma1. We found that the polytopic membrane protein Ist2 in the...
متن کاملMutations within an intramembrane leucine heptad repeat disrupt oligomer formation of the rat GABA transporter 1.
Na(+)/Cl(-)-dependent neurotransmitter transporters form constitutive oligomers, the significance of which is not known. In soluble proteins, leucine heptad repeats drive dimerization; the rat gamma-aminobutyric acid transporter GAT-1 (rGAT) contains a motif reminiscent of a leucine heptad repeat in the second transmembrane helix (TM2). We substituted leucine residues in TM2 of rGAT by alanine ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Soft matter
دوره 11 39 شماره
صفحات -
تاریخ انتشار 2015